RESUMO
Maintaining the integrity of the mitotic spindle in metaphase is essential to ensure normal cell division. We show here that depletion of microtubule-associated protein ATIP3 reduces metaphase spindle length. Mass spectrometry analyses identified the microtubule minus-end depolymerizing kinesin Kif2A as an ATIP3 binding protein. We show that ATIP3 controls metaphase spindle length by interacting with Kif2A and its partner Dda3 in an Aurora kinase A-dependent manner. In the absence of ATIP3, Kif2A and Dda3 accumulate at spindle poles, which is consistent with reduced poleward microtubule flux and shortening of the spindle. ATIP3 silencing also limits Aurora A localization to the poles. Transfection of GFP-Aurora A, but not kinase-dead mutant, rescues the phenotype, indicating that ATIP3 maintains Aurora A activity on the poles to control Kif2A targeting and spindle size. Collectively, these data emphasize the pivotal role of Aurora kinase A and its mutual regulation with ATIP3 in controlling spindle length.
Assuntos
Aurora Quinase A/genética , Cinesinas/genética , Fosfoproteínas/genética , Fuso Acromático/genética , Proteínas Supressoras de Tumor/genética , Células HeLa , Humanos , Metáfase , Proteínas Associadas aos Microtúbulos/genética , Microtúbulos/genética , Mitose/genéticaRESUMO
LINC complexes are transmembrane protein assemblies that physically connect the nucleoskeleton and cytoskeleton through the nuclear envelope. Dysfunctions of LINC complexes are associated with pathologies such as cancer and muscular disorders. The mechanical roles of LINC complexes are poorly understood. To address this, we used genetically encoded FRET biosensors of molecular tension in a nesprin protein of the LINC complex of fibroblastic and epithelial cells in culture. We exposed cells to mechanical, genetic, and pharmacological perturbations, mimicking a range of physiological and pathological situations. We show that nesprin experiences tension generated by the cytoskeleton and acts as a mechanical sensor of cell packing. Moreover, nesprin discriminates between inductions of partial and complete epithelial-mesenchymal transitions. We identify the implicated mechanisms, which involve α-catenin capture at the nuclear envelope by nesprin upon its relaxation, thereby regulating ß-catenin transcription. Our data thus implicate LINC complex proteins as mechanotransducers that fine-tune ß-catenin signaling in a manner dependent on the epithelial-mesenchymal transition program.
Assuntos
Transição Epitelial-Mesenquimal/genética , Mecanotransdução Celular/genética , Complexos Multiproteicos/genética , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , beta Catenina/genética , Animais , Técnicas Biossensoriais , Cães , Transferência Ressonante de Energia de Fluorescência , Humanos , Células Madin Darby de Rim Canino , Camundongos , Microtúbulos/genética , Células NIH 3T3 , Membrana Nuclear/genética , Matriz Nuclear/genéticaRESUMO
Predictive biomarkers for tumor response to neoadjuvant chemotherapy are needed in breast cancer. This study investigates the predictive value of 280 genes encoding proteins that regulate microtubule assembly and function. By analyzing 3 independent multicenter randomized cohorts of breast cancer patients, we identified 17 genes that are differentially regulated in tumors achieving pathological complete response (pCR) to neoadjuvant chemotherapy. We focused on the MTUS1 gene, whose major product, ATIP3, is a microtubule-associated protein down-regulated in aggressive breast tumors. We show here that low levels of ATIP3 are associated with an increased pCR rate, pointing to ATIP3 as a predictive biomarker of breast tumor chemosensitivity. Using preclinical models of patient-derived xenografts and 3-dimensional models of breast cancer cell lines, we show that low ATIP3 levels sensitize tumors to the effects of taxanes but not DNA-damaging agents. ATIP3 silencing improves the proapoptotic effects of paclitaxel and induces mitotic abnormalities, including centrosome amplification and multipolar spindle formation, which results in chromosome missegregation leading to aneuploidy. As shown by time-lapse video microscopy, ATIP3 depletion exacerbates cytokinesis failure and mitotic death induced by low doses of paclitaxel. Our results favor a mechanism by which the combination of ATIP3 deficiency and paclitaxel treatment induces excessive aneuploidy, which in turn results in elevated cell death. Together, these studies highlight ATIP3 as an important regulator of mitotic integrity and a useful predictive biomarker for a population of chemoresistant breast cancer patients.
Assuntos
Aneuploidia , Antineoplásicos Fitogênicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Resistencia a Medicamentos Antineoplásicos/genética , Proteínas de Neoplasias/fisiologia , Paclitaxel/farmacologia , Proteínas Supressoras de Tumor/fisiologia , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Citocinese/efeitos dos fármacos , DNA de Neoplasias/efeitos dos fármacos , Perfilação da Expressão Gênica , Xenoenxertos , Humanos , Microtúbulos/efeitos dos fármacos , Microtúbulos/fisiologia , Estudos Multicêntricos como Assunto/estatística & dados numéricos , Terapia Neoadjuvante , Invasividade Neoplásica/genética , Transplante de Neoplasias , Interferência de RNA , Ensaios Clínicos Controlados Aleatórios como Assunto/estatística & dados numéricos , Fuso Acromático/efeitos dos fármacos , Fuso Acromático/ultraestrutura , Taxoides/farmacologia , Imagem com Lapso de Tempo , Proteínas Supressoras de Tumor/antagonistas & inibidores , Proteínas Supressoras de Tumor/genéticaRESUMO
In epithelia, E-cadherin cytoplasmic tail is under cytoskeleton-generated tension via a link that contains ß-catenin. A cotranscription factor, ß-catenin, is also active in morphogenetic processes associated with epithelial-to-mesenchymal transition. ß-Catenin signaling appears mechanically inducible and was proposed to follow phosphorylation-induced ß-catenin release from E-cadherin. Evidence for this mechanism is lacking, and whether E-cadherin tension is involved is unknown. To test this, we combined quantitative fluorescence microscopies with genetic and pharmacological perturbations of epithelial-to-mesenchymal transition-induced cells in culture. We showed that ß-catenin nuclear activity follows a substantial release from the membrane specific to migrating cells and requires multicellular deconfinement and Src activity. Selective nuclear translocation occurs downstream of focal adhesion kinase activation, which targets E-cadherin tension relaxation through actomyosin remodeling. In contrast, phosphorylations of the cadherin/catenin complex are not substantially required. These data demonstrate that E-cadherin acts as a sensor of intracellular mechanics in a crosstalk with cell-substrate adhesions that target ß-catenin signaling.
